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ANTIMYCOBACTERIAL ACTIVITY OF KAPPAPHYCUS ALVAREZII AGAINST MYCOBACTERIUM TUBERCULOSIS AND IN SILICO MOLECULAR DOCKING OF KAPPA-CARRAGEENAN AGAINST InhA ENZYME

Abstract

A. Mayakrishnan1, K. Sivakumari1*, K. Ashok1, Prabhu Srinivasan2 and Vanaja Kumari2

In the present study, the antimycobacterial activity of Kappaphycus alvarezii against H37RV and MDR clinical isolate strains of Mycobacterium tuberculosis was carried out using three different solvent extracts (acetone, chloroform and ethanol) at two different concentrations (100 µg/ml and 500 µg/ml) by Luciferase Reporter Phage assay. The analysis depicted that 500 µg/ml of acetone and chloroform extracts have a significant antimycobacterial activity against H37RV strain of Mycobacterium tuberculosis. On the other hand, all the three extracts at both 100 µg/ml and 500 µg/ml have antimycobacterial activity against the clinical isolate strain of Mycobacterium tuberculosis. The in silico docking of kappa-carrageenan against the InhA enzyme were done by AutoDock Software and Accelrys Discovery Studio Visualization Tool and the docking studies found out that kappa-carrageenan formed one hydrogen bond with the docking score of -11.5 with InhA enzyme. This reveals that kappa-carrageenan has inhibitory activity against InhA enzyme, thus controlling the activity of Mycobacterium tuberculosis.  

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