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DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR THE ESTIMATION OF ANTIRETROVIRAL DRUGS AND THEIR PHARMACEUTICAL FORMULATIONS

Abstract

Naresh Chandra Joshi, Pradeep Kumar and Rakesh Kumar Jat

New, basic, delicate, exact & powerful superior dainty layer chromatographic (HPTLC) strategy was created for synchronous estimation of TENO & EMTRI in pharmaceutical measurement shapes. After improvement of plate, Camas TLC scanner III (examining speed 20 mm sec-1) was utilized for densitometry checking with WinCATs programming (opening miniaturized scale, 6 x 0.45 mm). Investigation of plate in absorbance mode at 274 nm was done for both medications all through trial. Framework was found to give smaller spots for TENO & EMTRI with RF (Retardation variable) estimation of 0.41±0.01and 0.56±0.01 separately. Data for adjustment plots demonstrated great direct association with r2 = 0.9985 & 0.9979 in fixation scope of 100-600ng/ml for both medications. Present technique was accepted by Conference on Harmonization (ICH) rules. Measurable investigation of information demonstrated that strategy is reproducible & exceptionally specific for estimation of TENO & EMTRI in quality control labs.

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