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EVALUATION OF PHENOLIC COMPOUNDS, FLAVONOIDS AND ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF METHANOLIC AND AQUEOUS LEAVES AND BRANCHES OF DAPHNE GNIDIUM L.

Abstract

Nouioua Wafaa* and Gaamoune Sofianeb

Daphne gnidium L. (Thymeleaceae) are evergreen shrubs native to Asia, Europe, and North Africa. Various species of Daphne are used in several folk medicines to treat gonorrhoea and cutaneous affections, rheumatoid arthritis, wound healings, malaria and anti-inflammations. The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of methanolic and aqueous leaves and branches of Daphne gnidium. Antioxidant activity was determined by DPPH free radical scavenging capacity and the reducing power activity, the antimicrobial activity was tested with three bacterial strain and one fungi including yeast (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC6633 and Candida albicans ATCC1024). Our results showed that the aqueous and methanolic leaves extract reported a considerable free radicals scavenging activity and reducing effect, because the extracts are richness on polyphenols and flavonoids.

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