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EXPRESSION AND PURIFICATION OF RECOMBINANT IFN-γ PROTEINS IN ESCHERICHIA COLI BL21

Abstract

Ishan Y. Pandya

Recombinant protein is a protein that it code was carried by recombinant DNA (r-DNA) i.e. two segments of DNA in plasmid. When r-DNA is inserted to bacteria, these bacteria will produce protein based on this recombinant DNA. This protein is called recombinant protein. The objective of present research study is to purify the recombinant protein from given unknown cellular culture. Molecular techniques like gene insertion, chemical transformation, Blue-white screening, Ultra-sonication, Electrophoresis purity test and Western blot identification test were followed. From the use of above molecular techniques, the recombinant IFN-γ was identified from Escherichia coli (strain BL-21). Present research paper also entails about the difficulties across during the production of recombinant IFN-γ proteins.

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