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PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITIES OF AQUEOUS EXTRACT OF INDIGOFERA TINCTORIA AND INDIGOFERA ASTRAGALINA

Abstract

R. Ramani Bai, M. Boothapandi, A. Madhavarani

The phytochemical screening of primary and secondary metabolites present in the plants use for various bioactive applications lead to the development of new novel plant based drugs. The present study aimed to address in vitro antioxidant activities of Indigofera tinctoria and Indigofera astragalina are commercially available and used in traditional medicine, because of its high nutritive value. The in vitro antioxidant study was carried out to study for its total reducing power, nitric oxide radicals scavenging, hydroxyl radicals scavenging, DPPH scavenging and total antioxidant activity. The results showed that the flavonoid and phenolic content of aqueous extracts of I. tinctoria and I. astragalina was 43.94 µg/mg, 55.05 µg/mg and 41.07 µg/mg, 36.55 µg/mg respectively. The IC50 value of I. tinctoria aqueous extract for DPPH, Nitric oxide radical scavenging and Hydroxyl radical scavenging was 512.66 ± 1.26 µg/mL, 585.28 ± 1.24 µg/mL and 483.78 ± 1.18 µg/mL. The IC50 value I. astragalina was 650.51 ± 1.20 µg/mL, 662.32 ± 1.26 µg/mL and 525.57 ± 1.28 µg/mL. The high absorbance of total reducing power and total antioxidant activity indicates a higher antioxidant activity of the extracts. In our knowledge aqueous extract of I .tinctoria and I. astragalina showed a potential antioxidant activity because of its high phenolic contents.

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