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STUDY OF DRUG-DRUG INTERACTION AND SIMULTANEOUS ESTIMATION OF A NEW COMBINATIONAL DRUG BY DSC AND HPLC

Abstract

Mohammad Farhadur Rahman, Muhammad Anisur Rahman, Md. Zakir Sultan and Md. Abdus Salam

It is a common practice in medical science to prescribe more than one drug. One drug may interact with another drug or foods which causes serious side effects in human health. In lieu of administrating individual drug it’s better to take developed combinational drug. This study observed the interaction of individual drugs in two homogeneous multi mixture A and B by DSC and HPLC at 1:1 molar ratio. A new RP-HPLC methodalso developed and validated for a combinational drug of valsartan and ciprofloxacin HCl. The DSC thermo gram of mixture-A (valsartan, naproxen, lercanidipine, cefepime and metformin HCl) and mixture-B (ramipril, naproxen, lercanidipine, ciprofloxacin HCl and gliclazide BP) shown sharp melting endotherm at 62.90°C, 104.05°C & 327.86°C and at 59.77°C & 105.51°C respectively. In HPLC, only one retention time found at 6.431 ± 0.1 for mixture-A and two retention time at 6.373 ± 0.1 and 7.196 ± 0.1 for mixture-B, under the condition of 2% acetic acid (PH 2.70) and acetonitrile (30:70, v/v) at 215nm and 254nm. But at same condition when the 5% acetic acid (PH 3.45) used, no sharp peaks observed for both mixtures. That indicates strong interactions due to bond breaking and forming among the drugs. Second,for simultaneous estimationof a combinational drug of ciprofloxacin HCl and valsartan, the retention times found at 2.770 ± 0.1 min and 8.647 ± 0.1 min, the correlation coefficient found at 0.9991 and 0.9993 and the percentage recovery found 98.48% and 101.70%, respectively, under the condition of 2% acetic acid (PH 2.70) and acetonitrile (40:60, v/v) at 240nm. The % RSD values found for ciprofloxacin HCl as 0.80-1.83 and valsartan as 0.41-1.33 by observing both intra and inter day.

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