International Journal of Drug Research and Technology

VALIDATED STABILITY INDICATING HPTLC METHOD FOR DIAZEPAM AND IMIPRAMINE IN BULK AND COMBINED DOSAGE FORM

Abstract

Vishal Srivastava, Pradeep Kumar, and Rakesh Kumar Jat

A Rapid, simple, specific, accurate and precise HPTLC method was developed for the simultaneous determination of Diazepam and Imipramine hydrochloride by using Silica gel 60 F254 precoated on aluminum sheet (10 cm × 10 cm) of 0.20 mm layer thickness (E. Merck KGaA) as stationary phase & Chloroform: Methanol: Hexane: Glacial Acetic Acid (3:3.5:3.5:0.2 v/v/v/v) as mobile phase. Camag Linomat 5 automatic application applicator, twin trough glass chamber, Camag TLC scanner and wincats software were used throughout the experiment. The Rf value were found to be 0.25 ± 0.01 for Imipramine hydrochloride & 0.47 ± 0.05 for diazepam (DIA) Respectively. Certification was done at 251 nm where Imipramine hydrochloride (IMI) & diazepam (DIA) have basic absorbance. The proposed method can be successfully employed for simultaneous quantitative analysis of Diazepam and Imipramine hydrochloride in bulk drugs and formulations.