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VALIDATED STABILITY INDICATING HPTLC METHOD FOR DIAZEPAM AND IMIPRAMINE IN BULK AND COMBINED DOSAGE FORM

Abstract

Vishal Srivastava, Pradeep Kumar, and Rakesh Kumar Jat

A Rapid, simple, specific, accurate and precise HPTLC method was developed for the simultaneous determination of Diazepam and Imipramine hydrochloride by using Silica gel 60 F254 precoated on aluminum sheet (10 cm × 10 cm) of 0.20 mm layer thickness (E. Merck KGaA) as stationary phase & Chloroform: Methanol: Hexane: Glacial Acetic Acid (3:3.5:3.5:0.2 v/v/v/v) as mobile phase. Camag Linomat 5 automatic application applicator, twin trough glass chamber, Camag TLC scanner and wincats software were used throughout the experiment. The Rf value were found to be 0.25 ± 0.01 for Imipramine hydrochloride & 0.47 ± 0.05 for diazepam (DIA) Respectively. Certification was done at 251 nm where Imipramine hydrochloride (IMI) & diazepam (DIA) have basic absorbance. The proposed method can be successfully employed for simultaneous quantitative analysis of Diazepam and Imipramine hydrochloride in bulk drugs and formulations.

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