+447897072958Saidu Ibrahim Ngulde
Department of Veterinary Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Maiduguri,, NigeriaPublications
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Original Research Article
ANTITRYPANOSOMAL POTENCY OF METHANOL EXTRACT OF CASSIA AREREH DELILE ROOT BARK IN ALBINO RATS
Author(s): Saidu Ibrahim Ngulde
ABSTRACT The methanol extract of the root bark of Cassia arereh Del. was investigated for antitrypanosomal activity in vitro and in vivo using Trypanosoma brucei brucei. In vitro antritrypanosomal potency was performed in triplicate in 96 wells microtitre plate, where the infected blood was treated with 1.25, 2.5, 5 and 10mg/ml concentration of plant extract and also with 3.5mg/ml diminazene aceturate and with dextrose saline. The oral lethal dose (LD50) was determined using Lorke’s method. Rats were inoculated with T. brucei brucei intraperitonealy and treated with the extract orally at 500 and 1000mg/ml (group A and B) while group C and D were given a single dose of 3.5mg/kg of diminazene aceturate and 0.5ml distilled water, respectively. In the in vitro study, there was significant extract concentration dependent activity against the parasite motility. The in vivo study revea.. Read More»